starfish: scalable pipelines for image-based transcriptomics
starfish is a Python library for processing images of image-based spatial transcriptomics. It lets you build scalable pipelines that localize and quantify RNA transcripts in image data generated by any FISH method, from simple RNA single-molecule FISH to combinatorial barcoded assays.
If you want to give it a try, the quick start tutorial will guide you from installation to running a pipeline in under 10 minutes. For more comprehensive instructions on how to use starfish, see the user guide on creating image-based transcriptomics processing pipelines, which organizes and contextualizes the tutorials on running starfish using the API. Finally, advanced users can examine the Data Structures and Help & Reference sections to learn more details about starfish and its object models.
In addition to the library of image processing functions, starfish introduces a standardized data format for image-based spatial transcriptomic assays. Examples and tutorials for formatting your data into the SpaceTx format can be found in Formatting Data.